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Vitamin
Analysis by HPLC |
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- Introduction
Of the HPLC application in analysis of Vitamins, the main problem
to be overcome is sample pretreatment other than the separation
from each other. If the Vitamins components are well extracted
or prepurified, they can be separated and quantified with the
most common HPLC system.
However, because of the fact that many Vitamins components in
various samples are from artificial and added afterwards, the
amounts of the Vitamins added are much superior to that from natural.
And therefore the analytical procedures can be greatly shortened
and simplified.
In this work, three methods of pretreatments were described according
different samples. And, using a non gradient chromatographic profile,
the fat soluble Vitamins in fish oil, plant oil, milk and make
up capsule were determined respectively.
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- Fat Soluable Vitamins
in Fish Oil
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Chromatogram of Standard
Fat Soluable Vitamins
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Analysis of Fat Soluable
Vitamins in Fish Oil
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- Fast Analysis of
Isomers of Vitamin E
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Chromatogram of Standard
Fat Soluable Vitamins
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- Analysis Vitamin
E in Plant Oils
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Vitamin E contents in
plant oils
| Sample |
Total Tocopherol
(mg/g) |
Vitamin
E (mg/g) |
| Oil of
Evening Primrose |
2.53 |
0.97 |
| Oil of
wheat germ |
14.76 |
1.86 |
| Oil of
Walnut |
15.93 |
8.25 |
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- Analysis of Vitamin
E in Milk
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Vitamin
E contents in Intensified Milk (Dalian dairy products factory)
| Sample |
Vitamin
E (mg/g) |
| Common
Milk |
14.1 |
| Intensified
Milk |
20.5 |
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