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Affinity
Chromatography based on Modified Sepharose 4B |
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- Introduction
A method for the preparation of an p-aminobenzene sulphonyl ethyl
containing crosslinked Sepharose 4B (ABSE-Sepharose 4B-CL) is
described. Trypsin, Bovine Serum Albumin(BSA) and Concanavalin
A (Con A) were immobilized onto this matrix by diazotization.
Conditions for the coupling reaction were investigated. The activity
of immobilized trypsin reached up to 1.4*104 U/g and 25 mg BSA
can be coupled onto 1 g ABSE-Sepharose 4B-CL under the optimal
conditions. An affinity medium immobilized Con A as ligand was
prepared by this method, and was used in the separation of a human
monoclonal antibody.
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- Separation of Monoclonal
Antibody
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- Separation of gamma-Interferon
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- Separation of alpha-Interferon
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